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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
19/12/2017 |
Data da última atualização: |
21/05/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
REIS, A. N. dos; MIRANDA, M. dos S.; SILVA, L. K. X.; SILVA, A. O. A. da; SOUSA, J. S. de; MORAIS, E. de; GARCIA, A. R.; DOMINGUES, S. F. S.; SILVA, J. K. do R. da; RIBEIRO, H. F. L. |
Afiliação: |
Adriana Novaes dos Reis, UFPA; Moysés dos Santos Miranda, UFPA; Lílian Kátia Ximenes Silva, UNAMA; Aluizio Otavio Almeida da Silva, UFPA; José Silva de Sousa, UFPA; Eziquiel de Morais, IFPA; ALEXANDRE ROSSETTO GARCIA, CPPSE; Sheyla Farhayldes Souza Domingues, UFPA; Joyce Kelly do Rosário da Silva, UFPA; Haroldo Francisco Lobato Ribeiro, UFRA. |
Título: |
Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 38, n. 6, p. 3613-3628, 2017. |
DOI: |
10.5433/1679-0359.2017v38n6p3613 |
Idioma: |
Inglês |
Conteúdo: |
For artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil. MenosFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evalu... Mostrar Tudo |
Palavras-Chave: |
Powdered Coconut Water. |
Thesaurus Nal: |
cryopreservation; Lippia. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169304/1/REIS-et-al-2016-Comparative-Evaluation-Between-TES-TRIS-and-ACP-112.pdf
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Marc: |
LEADER 03425naa a2200277 a 4500 001 2082949 005 2019-05-21 008 2017 bl uuuu u00u1 u #d 024 7 $a10.5433/1679-0359.2017v38n6p3613$2DOI 100 1 $aREIS, A. N. dos 245 $aComparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm.$h[electronic resource] 260 $c2017 520 $aFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil. 650 $acryopreservation 650 $aLippia 653 $aPowdered Coconut Water 700 1 $aMIRANDA, M. dos S. 700 1 $aSILVA, L. K. X. 700 1 $aSILVA, A. O. A. da 700 1 $aSOUSA, J. S. de 700 1 $aMORAIS, E. de 700 1 $aGARCIA, A. R. 700 1 $aDOMINGUES, S. F. S. 700 1 $aSILVA, J. K. do R. da 700 1 $aRIBEIRO, H. F. L. 773 $tSemina: Ciências Agrárias, Londrina$gv. 38, n. 6, p. 3613-3628, 2017.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registros recuperados : 8 | |
2. | | SILVA, L. K. X. da S.; GARCIA, A. R.; MARTORANO, L. G.; FRANCO, I. M.; SILVA, A. O. A. da; SOUSA, J. S.; BARROS, D. V.; FATURI, C. Scrotum surface infrared thermography associated with semen quality of buffaloes (Bubalus bubalis). In: INTERNATIONAL ECOSUMMIT, 5., 2016, Montpellier, France. Proceedings... Montpellier, France: ESEC, 2016. p. 334.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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3. | | SILVA, L. K. X.; GARCIA, A. R.; SILVA, A. O. A. da; SOUSA, J. S. de; BARROS, D. V.; SILVA, A. G. M. e; REIS, A. N. dos; MARTORANO, L. G.; LOUREÇO JÚNIOR, J. de B. Correlações entre parâmetros seminais de touros bubalinos em central de inseminação artificial. In: CONGRESSO BRASILEIRO DE BUIATRIA, 10.; SEMANA DO MÉDICO VETERINÁRIO DO PARÁ, 37.; SIMPÓSIO PARAENSE DE MEDICINA VETERINÁRIA, 5., 2013, Belém. Anais... Belém: Associação Brasileira de Buiatria, 2013 1 CD-ROMTipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Pecuária Sudeste. |
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4. | | GARCIA, A. R.; SILVA, L. K. X.; BARROS, D. V.; LOURENÇO JUNIOR, J. de B.; MARTORANO, L. G.; LISBOA, L. S. S.; SILVA, J. A. R. da; SOUSA, J. S. de; SILVA, A. O. A. da. Key points for the thermal comfort of water buffaloes in Eastern Amazon. Ciência Rural, Santa Maria, v. 53, n. 1, e20210544, 2023.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Pecuária Sudeste. |
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5. | | MARQUES, J. R. F.; RIBEIRO, H. F. L.; COSTA, M. R.; SILVA, A. O. A. da; SOUZA, J. S. de; COSTA, N. A. da; MARQUES, L. C.; CAMARGO JÚNIOR, R. N. C.; ROCHA, A. R. da. Eficiência reprodutiva e melhoramento genética de bubalinos e bovinos. In: MARQUES, J. R. F.; LOPES, C. A. C.; MARTINEZ, G. B. (Ed.). Produção animal nas várzeas do Rio Amazonas. Belém, PA: Embrapa Amazônia Oriental, 2003. p. 303-323.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Amazônia Oriental. |
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6. | | BARROS, D. V.; SILVA, L. K. X.; LOURENÇO JUNIOR, J. de B.; SILVA, A. O. A. da; SILVA, A. G. M. e; FRANCO, I. M.; OLIVEIRA, C. M. C.; THOLON, P.; MARTORANO, L. G.; GARCIA, A. R. Evaluation of thermal comfort, physiological, hematological, and seminal features of buffalo bulls in an artificial insemination station in a tropical environment. Tropical Animal Health and Production, v. 47, n.5, p. 805-813, june 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Pecuária Sudeste. |
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7. | | SILVA, L. K. X.; BARROS, D. V.; LOURENÇO JUNIOR, J. de B.; SILVA, A. O. A. da; SOUSA, J. S. de; FATURI, C.; PANTOJA, M. H. de A.; BOTTA, D.; GARCIA, A. R. Temperaturas de superfície escrotal avaliadas por termografia de infravermelho e qualidade seminal de touros bubalinos em condições de clima tropical úmido. In: REUNIÃO DA ASSOCIAÇÃO BRASILEIRA DE ANDROLOGIA ANIMAL, 2., 2017, Uberlândia, MG. Anais... Corumbá: Embrapa Pantanal, 2017. p. 187-190. (Embrapa Pantanal. Documentos, 146)Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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8. | | REIS, A. N. dos; MIRANDA, M. dos S.; SILVA, L. K. X.; SILVA, A. O. A. da; SOUSA, J. S. de; MORAIS, E. de; GARCIA, A. R.; DOMINGUES, S. F. S.; SILVA, J. K. do R. da; RIBEIRO, H. F. L. Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm. Semina: Ciências Agrárias, Londrina, v. 38, n. 6, p. 3613-3628, 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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